Complementary surface-enhanced resonance raman spectroscopic biodetection of mixed protein solutions by chitosan- and silica-coated plasmon-tuned silver nanoparticles
Research output: Contribution to journal › Research article › Contributed › peer-review
Contributors
Abstract
Silver nanoparticles with identical plasmonic properties but different surface functionalities are synthesized and tested as chemically selective surface-enhanced resonance Raman (SERR) amplifiers in a two-component protein solution. The surface plasmon resonances of the particles are tuned to 413 nm to match the molecular resonance of protein heme cofactors. Biocompatible functionalization of the nanoparticles with a thin film of chitosan yields selective SERR enhancement of the anionic protein cytochrome b5, whereas functionalization with SiO2 amplifies only the spectra of the cationic protein cytochrome c. As a result, subsequent addition of the two differently functionalized particles yields complementary information on the same mixed protein sample solution. Finally, the applicability of chitosan-coated Ag nanoparticles for protein separation was tested by in situ resonance Raman spectroscopy.
Details
Original language | English |
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Pages (from-to) | 5759-5764 |
Number of pages | 6 |
Journal | Analytical Chemistry |
Volume | 84 |
Issue number | 13 |
Publication status | Published - 3 Jul 2012 |
Peer-reviewed | Yes |
Externally published | Yes |
External IDs
PubMed | 22690823 |
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