Complementary surface-enhanced resonance raman spectroscopic biodetection of mixed protein solutions by chitosan- and silica-coated plasmon-tuned silver nanoparticles
Publikation: Beitrag in Fachzeitschrift › Forschungsartikel › Beigetragen › Begutachtung
Beitragende
Abstract
Silver nanoparticles with identical plasmonic properties but different surface functionalities are synthesized and tested as chemically selective surface-enhanced resonance Raman (SERR) amplifiers in a two-component protein solution. The surface plasmon resonances of the particles are tuned to 413 nm to match the molecular resonance of protein heme cofactors. Biocompatible functionalization of the nanoparticles with a thin film of chitosan yields selective SERR enhancement of the anionic protein cytochrome b5, whereas functionalization with SiO2 amplifies only the spectra of the cationic protein cytochrome c. As a result, subsequent addition of the two differently functionalized particles yields complementary information on the same mixed protein sample solution. Finally, the applicability of chitosan-coated Ag nanoparticles for protein separation was tested by in situ resonance Raman spectroscopy.
Details
Originalsprache | Englisch |
---|---|
Seiten (von - bis) | 5759-5764 |
Seitenumfang | 6 |
Fachzeitschrift | Analytical Chemistry |
Jahrgang | 84 |
Ausgabenummer | 13 |
Publikationsstatus | Veröffentlicht - 3 Juli 2012 |
Peer-Review-Status | Ja |
Extern publiziert | Ja |
Externe IDs
PubMed | 22690823 |
---|