Characterisation of lipofuscin-like lysosomal inclusion bodies from human placenta
Research output: Contribution to journal › Research article › Contributed › peer-review
Contributors
Abstract
A structural hallmark of lysosomes is heterogeneity of their contents. We describe a method for isolation of particulate materials from human placental lysosomes. After a methionine methyl ester-induced disruption of lysosomes and two density gradient centrifugations we obtained a homogeneous membrane fraction and another one enriched in particulate inclusions. The latter exhibited a yellow-brown coloration and contained bodies lacking a delimiting membrane, which were characterised by a granular pattern and high electron density. The lipofuscin-like inclusion materials were rich in tripeptidyl peptidase I, β-glucuronidase, acid ceramidase and apolipoprotein D and contained proteins originating from diverse subcellular localisations. Here we show that human term placenta contains lipofuscin-like lysosomal inclusions, a phenomenon usually associated with senescence in postmitotic cells. These findings imply that a simple pelleting of a lysosomal lysate is not appropriate for the isolation of lysosomal membranes, as the inclusions tend to be sedimented with the membranes.
Details
Original language | English |
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Pages (from-to) | 102-108 |
Number of pages | 7 |
Journal | FEBS letters |
Volume | 581 |
Issue number | 1 |
Publication status | Published - 9 Jan 2007 |
Peer-reviewed | Yes |
External IDs
PubMed | 17174955 |
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Keywords
ASJC Scopus subject areas
Keywords
- Ageing, Lipofuscin, Lysosomal membrane, Placenta, Tripeptidyl peptidase I