Characterisation of lipofuscin-like lysosomal inclusion bodies from human placenta
Publikation: Beitrag in Fachzeitschrift › Forschungsartikel › Beigetragen › Begutachtung
Beitragende
Abstract
A structural hallmark of lysosomes is heterogeneity of their contents. We describe a method for isolation of particulate materials from human placental lysosomes. After a methionine methyl ester-induced disruption of lysosomes and two density gradient centrifugations we obtained a homogeneous membrane fraction and another one enriched in particulate inclusions. The latter exhibited a yellow-brown coloration and contained bodies lacking a delimiting membrane, which were characterised by a granular pattern and high electron density. The lipofuscin-like inclusion materials were rich in tripeptidyl peptidase I, β-glucuronidase, acid ceramidase and apolipoprotein D and contained proteins originating from diverse subcellular localisations. Here we show that human term placenta contains lipofuscin-like lysosomal inclusions, a phenomenon usually associated with senescence in postmitotic cells. These findings imply that a simple pelleting of a lysosomal lysate is not appropriate for the isolation of lysosomal membranes, as the inclusions tend to be sedimented with the membranes.
Details
Originalsprache | Englisch |
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Seiten (von - bis) | 102-108 |
Seitenumfang | 7 |
Fachzeitschrift | FEBS letters |
Jahrgang | 581 |
Ausgabenummer | 1 |
Publikationsstatus | Veröffentlicht - 9 Jan. 2007 |
Peer-Review-Status | Ja |
Externe IDs
PubMed | 17174955 |
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Schlagworte
ASJC Scopus Sachgebiete
Schlagwörter
- Ageing, Lipofuscin, Lysosomal membrane, Placenta, Tripeptidyl peptidase I