Axolotl Transgenesis via Injection of I-SceI Meganuclease or Tol2 Transposon System
Research output: Contribution to book/conference proceedings/anthology/report › Chapter in book/anthology/report › Invited
Abstract
The axolotl (Ambystoma mexicanum ) has been widely used as an animal model for studying development and regeneration. In recent decades, the use of genetic engineering to alter gene expression has advanced our knowledge on the fundamental molecular and cellular mechanisms, pointing us to potential therapeutic targets. We present a detailed, step-by-step protocol for axolotl transgenesis using either I-SceI meganuclease or the mini Tol2 transposon system, by injection of purified DNA into one-cell stage eggs. We add useful tips on the site of injection and the viability of the eggs.
Details
| Original language | English |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Pages | 321-333 |
| Number of pages | 13 |
| Volume | 2562 |
| Publication status | Published - 2023 |
| Peer-reviewed | No |
Publication series
| Series | Methods in Molecular Biology (MIMB; Vol. 2562) |
|---|---|
| Volume | 2562 |
| ISSN | 1064-3745 |
External IDs
| Scopus | 85140350918 |
|---|---|
| unpaywall | 10.1007/978-1-0716-2659-7_21 |
| Mendeley | dcf0afe8-d5c6-3619-a374-ff941cb90a33 |
Keywords
Research priority areas of TU Dresden
DFG Classification of Subject Areas according to Review Boards
ASJC Scopus subject areas
Keywords
- Animals, Ambystoma mexicanum/genetics, Deoxyribonucleases, Type II Site-Specific/metabolism, Gene Transfer Techniques, DNA/genetics, Injections, I-SceI meganuclease, Axolotl eggs, mTol2 transposase, Egg injection, Transgenesis