Axolotl Transgenesis via Injection of I-SceI Meganuclease or Tol2 Transposon System

Research output: Contribution to book/conference proceedings/anthology/reportChapter in book/anthology/reportInvited



The axolotl (Ambystoma mexicanum ) has been widely used as an animal model for studying development and regeneration. In recent decades, the use of genetic engineering to alter gene expression has advanced our knowledge on the fundamental molecular and cellular mechanisms, pointing us to potential therapeutic targets. We present a detailed, step-by-step protocol for axolotl transgenesis using either I-SceI meganuclease or the mini Tol2 transposon system, by injection of purified DNA into one-cell stage eggs. We add useful tips on the site of injection and the viability of the eggs.


Original languageEnglish
Title of host publicationMethods in Molecular Biology
Number of pages13
Publication statusPublished - 2023

Publication series

SeriesMethods in molecular biology (Clifton, N.J.)

External IDs

Scopus 85140350918
unpaywall 10.1007/978-1-0716-2659-7_21
Mendeley dcf0afe8-d5c6-3619-a374-ff941cb90a33


Research priority areas of TU Dresden

DFG Classification of Subject Areas according to Review Boards

ASJC Scopus subject areas


  • Animals, Ambystoma mexicanum/genetics, Deoxyribonucleases, Type II Site-Specific/metabolism, Gene Transfer Techniques, DNA/genetics, Injections, I-SceI meganuclease, Axolotl eggs, mTol2 transposase, Egg injection, Transgenesis