The axolotl (Ambystoma mexicanum ) has been widely used as an animal model for studying development and regeneration. In recent decades, the use of genetic engineering to alter gene expression has advanced our knowledge on the fundamental molecular and cellular mechanisms, pointing us to potential therapeutic targets. We present a detailed, step-by-step protocol for axolotl transgenesis using either I-SceI meganuclease or the mini Tol2 transposon system, by injection of purified DNA into one-cell stage eggs. We add useful tips on the site of injection and the viability of the eggs.
|Title of host publication||Methods in Molecular Biology|
|Number of pages||13|
|Publication status||Published - 2023|
|Series||Methods in molecular biology (Clifton, N.J.)|
Research priority areas of TU Dresden
- Animals, Ambystoma mexicanum/genetics, Deoxyribonucleases, Type II Site-Specific/metabolism, Gene Transfer Techniques, DNA/genetics, Injections, I-SceI meganuclease, Axolotl eggs, mTol2 transposase, Egg injection, Transgenesis