Axolotl Transgenesis via Injection of I-SceI Meganuclease or Tol2 Transposon System

Publikation: Beitrag in Buch/Konferenzbericht/Sammelband/GutachtenBeitrag in Buch/Sammelband/GutachtenEingeladen

Beitragende

Abstract

The axolotl (Ambystoma mexicanum ) has been widely used as an animal model for studying development and regeneration. In recent decades, the use of genetic engineering to alter gene expression has advanced our knowledge on the fundamental molecular and cellular mechanisms, pointing us to potential therapeutic targets. We present a detailed, step-by-step protocol for axolotl transgenesis using either I-SceI meganuclease or the mini Tol2 transposon system, by injection of purified DNA into one-cell stage eggs. We add useful tips on the site of injection and the viability of the eggs.

Details

OriginalspracheEnglisch
TitelMethods in Molecular Biology
Seiten321-333
Seitenumfang13
Band2562
PublikationsstatusVeröffentlicht - 2023
Peer-Review-StatusNein

Publikationsreihe

ReiheMethods in Molecular Biology (MIMB; Vol. 2562)
Band2562
ISSN1064-3745

Externe IDs

Scopus 85140350918
unpaywall 10.1007/978-1-0716-2659-7_21
Mendeley dcf0afe8-d5c6-3619-a374-ff941cb90a33
ORCID /0000-0003-1802-5145/work/166764647

Schlagworte

Forschungsprofillinien der TU Dresden

DFG-Fachsystematik nach Fachkollegium

ASJC Scopus Sachgebiete

Schlagwörter

  • Animals, Ambystoma mexicanum/genetics, Deoxyribonucleases, Type II Site-Specific/metabolism, Gene Transfer Techniques, DNA/genetics, Injections, I-SceI meganuclease, Axolotl eggs, mTol2 transposase, Egg injection, Transgenesis