Axolotl Transgenesis via Injection of I-SceI Meganuclease or Tol2 Transposon System
Publikation: Beitrag in Buch/Konferenzbericht/Sammelband/Gutachten › Beitrag in Buch/Sammelband/Gutachten › Eingeladen
Beitragende
Abstract
The axolotl (Ambystoma mexicanum ) has been widely used as an animal model for studying development and regeneration. In recent decades, the use of genetic engineering to alter gene expression has advanced our knowledge on the fundamental molecular and cellular mechanisms, pointing us to potential therapeutic targets. We present a detailed, step-by-step protocol for axolotl transgenesis using either I-SceI meganuclease or the mini Tol2 transposon system, by injection of purified DNA into one-cell stage eggs. We add useful tips on the site of injection and the viability of the eggs.
Details
Originalsprache | Englisch |
---|---|
Titel | Methods in Molecular Biology |
Seiten | 321-333 |
Seitenumfang | 13 |
Band | 2562 |
Publikationsstatus | Veröffentlicht - 2023 |
Peer-Review-Status | Nein |
Publikationsreihe
Reihe | Methods in Molecular Biology (MIMB; Vol. 2562) |
---|---|
Band | 2562 |
ISSN | 1064-3745 |
Externe IDs
Scopus | 85140350918 |
---|---|
unpaywall | 10.1007/978-1-0716-2659-7_21 |
Mendeley | dcf0afe8-d5c6-3619-a374-ff941cb90a33 |
ORCID | /0000-0003-1802-5145/work/166764647 |
Schlagworte
Forschungsprofillinien der TU Dresden
DFG-Fachsystematik nach Fachkollegium
ASJC Scopus Sachgebiete
Schlagwörter
- Animals, Ambystoma mexicanum/genetics, Deoxyribonucleases, Type II Site-Specific/metabolism, Gene Transfer Techniques, DNA/genetics, Injections, I-SceI meganuclease, Axolotl eggs, mTol2 transposase, Egg injection, Transgenesis