Automated 10-channel capillary chip immunodetector for biological agents detection
Research output: Contribution to journal › Research article › Contributed › peer-review
Contributors
Abstract
The automated 10-channel capillary chip immunodetector (10K-IDWG) is a prototype, which has been developed for automatically operated biological agents (BA) point detection. The current technology uses a chemiluminescence capillary immunoassay (EIA) technique in combination with integrated microfluidics and allows the highly sensitive and rapid detection and preliminary identification of multiple BA in aqueous solutions in the laboratory. The chemiluminescence capillary EIA are performed within a disposable capillary chip containing 10 fused-silica capillaries arranged in parallel coated with selected capture antibodies. A multianode-photomultiplier array is used to detect chemiluminescence intensity in each capillary. Reservoirs for reagents and buffers and a waste disposal reservoir are integrated. This paper describes the technology of the 10K-IDWG and its evaluation with three different BA, the toxin staphylococcal enterotoxin B (SEB), the bacterial analyte Escherichia coli (E. coli) O157:H7 as a model for bacterial pathogens, and the bacteriophage M13 as a model for virus pathogens. The 10K-IDWG is able to detect the above mentioned three BA in an aqueous sample within 29 min (single analyte-detection and multiplexing). Limits of detection (LOD) are 0.1 ng/ml for SEB, 104 cfu/ml for E. coli O157:H7, and 5 × 105 pfu/ml for M13. Cross reactivities between the three assays were not observed.
Details
Original language | English |
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Pages (from-to) | 1368-1375 |
Number of pages | 8 |
Journal | Biosensors and Bioelectronics |
Volume | 22 |
Issue number | 7 |
Publication status | Published - 15 Feb 2007 |
Peer-reviewed | Yes |
External IDs
PubMed | 16839755 |
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ORCID | /0000-0002-0757-3325/work/139064969 |
Keywords
ASJC Scopus subject areas
Keywords
- Biological agent, Biosensor, Capillary enzyme immunoassay, Chemiluminescence, Microfluidics, Multiplex detection