Astin C production by the endophytic fungus Cyanodermella asteris in planktonic and immobilized culture conditions

Research output: Contribution to journalResearch articleContributedpeer-review


  • Antoine Vassaux - , University of Liege (Author)
  • Cédric Tarayre - , University of Liege (Author)
  • Anthony Arguëlles-Arias - , University of Liege (Author)
  • Philippe Compère - , University of Liege (Author)
  • Frank Delvigne - , University of Liege (Author)
  • Patrick Fickers - , University of Liege (Author)
  • Linda Jahn - , Chair of Plant Physiology (Author)
  • Alexander Lang - , Chair of Biochemistry (Author)
  • Valérie Leclère - , Université de Lille (Author)
  • Jutta Ludwig-Müller - , Chair of Plant Physiology (Author)
  • Marc Ongena - , University of Liege (Author)
  • Thomas Schafhauser - , University of Tübingen (Author)
  • Samuel Telek - , University of Liege (Author)
  • Ariane Théatre - , University of Liege (Author)
  • Willem J H van Berkel - , Wageningen University & Research (WUR) (Author)
  • Micheline Vandenbol - , University of Liege (Author)
  • Karl-Heinz van Pée - , Chair of Biochemistry (Author)
  • Luc Willems - , University of Liege (Author)
  • Wolfgang Wohlleben - , University of Tübingen (Author)
  • Philippe Jacques - , University of Liege (Author)


The fungal endophyte Cyanodermella asteris (C. asteris) has been recently isolated from the medicinal plant Aster tataricus (A. tataricus). This fungus produces astin C, a cyclic pentapeptide with anticancer and anti-inflammatory properties. The production of this secondary metabolite is compared in immobilized and planktonic conditions. For immobilized cultures, a stainless steel packing immersed in the culture broth is used as a support. In these conditions, the fungus exclusively grows on the packing, which provides a considerable advantage for astin C recovery and purification. C. asteris metabolism is different according to the culture conditions in terms of substrate consumption rate, cell growth, and astin C production. Immobilized-cell cultures yield a 30% increase of astin C production, associated with a 39% increase in biomass. The inoculum type as spores rather than hyphae, and a pre-inoculation washing procedure with sodium hydroxide, turns out to be beneficial both for astin C production and fungus development onto the support. Finally, the influence of culture parameters such as pH and medium composition on astin C production is evaluated. With optimized culture conditions, astin C yield is further improved reaching a five times higher final specific yield compared to the value reported with astin C extraction from A. tataricus (0.89 mg g-1 and 0.16 mg g-1 respectively).


Original languageEnglish
Pages (from-to)e1800624
JournalBiotechnology Journal
Issue number8
Publication statusPublished - Aug 2019

External IDs

Scopus 85069863331
ORCID /0000-0001-9147-4188/work/142257658



  • Ascomycota/cytology, Bioreactors, Cells, Immobilized, Culture Media/chemistry, Endophytes/metabolism, Industrial Microbiology/instrumentation, Peptides, Cyclic/biosynthesis, Plankton, Stainless Steel