Astin C production by the endophytic fungus Cyanodermella asteris in planktonic and immobilized culture conditions
Publikation: Beitrag in Fachzeitschrift › Forschungsartikel › Beigetragen › Begutachtung
Beitragende
Abstract
The fungal endophyte Cyanodermella asteris (C. asteris) has been recently isolated from the medicinal plant Aster tataricus (A. tataricus). This fungus produces astin C, a cyclic pentapeptide with anticancer and anti-inflammatory properties. The production of this secondary metabolite is compared in immobilized and planktonic conditions. For immobilized cultures, a stainless steel packing immersed in the culture broth is used as a support. In these conditions, the fungus exclusively grows on the packing, which provides a considerable advantage for astin C recovery and purification. C. asteris metabolism is different according to the culture conditions in terms of substrate consumption rate, cell growth, and astin C production. Immobilized-cell cultures yield a 30% increase of astin C production, associated with a 39% increase in biomass. The inoculum type as spores rather than hyphae, and a pre-inoculation washing procedure with sodium hydroxide, turns out to be beneficial both for astin C production and fungus development onto the support. Finally, the influence of culture parameters such as pH and medium composition on astin C production is evaluated. With optimized culture conditions, astin C yield is further improved reaching a five times higher final specific yield compared to the value reported with astin C extraction from A. tataricus (0.89 mg g-1 and 0.16 mg g-1 respectively).
Details
Originalsprache | Englisch |
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Seiten (von - bis) | e1800624 |
Fachzeitschrift | Biotechnology Journal |
Jahrgang | 14 |
Ausgabenummer | 8 |
Publikationsstatus | Veröffentlicht - Aug. 2019 |
Peer-Review-Status | Ja |
Externe IDs
Scopus | 85069863331 |
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ORCID | /0000-0001-9147-4188/work/142257658 |
Schlagworte
Schlagwörter
- Ascomycota/cytology, Bioreactors, Cells, Immobilized, Culture Media/chemistry, Endophytes/metabolism, Industrial Microbiology/instrumentation, Peptides, Cyclic/biosynthesis, Plankton, Stainless Steel