Active Loop Extrusion Guides DNA-Protein Condensation

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • Ryota Takaki - , Max-Planck-Institute for the Physics of Complex Systems (Author)
  • Yahor Savich - , Max-Planck-Institute for the Physics of Complex Systems, Max Planck Institute of Molecular Cell Biology and Genetics, Center for Systems Biology Dresden (CSBD) (Author)
  • Jan Brugués - , Clusters of Excellence PoL: Physics of Life, Max-Planck-Institute for the Physics of Complex Systems, Max Planck Institute of Molecular Cell Biology and Genetics, Center for Systems Biology Dresden (CSBD) (Author)
  • Frank Jülicher - , Max-Planck-Institute for the Physics of Complex Systems, Center for Systems Biology Dresden (CSBD), TUD Dresden University of Technology (Author)

Abstract

The spatial organization of DNA involves DNA loop extrusion and the formation of protein-DNA condensates. While the significance of each process is increasingly recognized, their interplay remains unexplored. Using molecular dynamics simulation and theory we investigate this interplay. Our findings reveal that loop extrusion can enhance the dynamics of condensation and promotes coalescence and ripening of condensates. Further, the DNA loop enables condensate formation under DNA tension and position condensates. The concurrent presence of loop extrusion and condensate formation results in the formation of distinct domains similar to TADs, an outcome not achieved by either process alone.

Details

Original languageEnglish
Article number128401
Number of pages7
JournalPhysical review letters
Volume134
Issue number12
Publication statusPublished - 28 Mar 2025
Peer-reviewedYes

Keywords

ASJC Scopus subject areas