V-ATPase inhibition increases cancer cell stiffness and blocks membrane related Ras signaling - A new option for HCC therapy

Research output: Contribution to journalResearch articleContributedpeer-review


  • Karin Bartel - (Author)
  • Maria Winzi - , Chair of Cellular Machines (Author)
  • Melanie Ulrich - (Author)
  • Andreas Koeberle - (Author)
  • Dirk Menche - (Author)
  • Oliver Werz - (Author)
  • Rolf Müller - (Author)
  • Jochen Guck - , Chair of Cellular Machines (Author)
  • Angelika M. Vollmar - (Author)
  • Karin von Schwarzenberg - (Author)


Hepatocellular carcinoma (HCC) is the fifth most frequent cancer worldwide and the third leading cause of cancer-related death. However, therapy options are limited leaving an urgent need to develop new strategies. Currently, targeting cancer cell lipid and cholesterol metabolism is gaining interest especially regarding HCC. High cholesterol levels support proliferation, membrane-related mitogenic signaling and increase cell softness, leading to tumor progression, malignancy and invasive potential. However, effective ways to target cholesterol metabolism for cancer therapy are still missing. The V-ATPase inhibitor archazolid was recently shown to interfere with cholesterol metabolism. In our study, we report a novel therapeutic potential of V-ATPase inhibition in HCC by altering the mechanical phenotype of cancer cells leading to reduced proliferative signaling. Archazolid causes cellular depletion of free cholesterol leading to an increase in cell stiffness and membrane polarity of cancer cells, while hepatocytes remain unaffected. The altered membrane composition decreases membrane fluidity and leads to an inhibition of membrane-related Ras signaling resulting decreased proliferation in vitro and in vivo. V-ATPase inhibition represents a novel link between cell biophysical properties and proliferative signaling selectively in malignant HCC cells, providing the basis for an attractive and innovative strategy against HCC.


Original languageEnglish
Pages (from-to)9476-9487
Number of pages12
Issue number6
Publication statusPublished - 2017


Sustainable Development Goals

ASJC Scopus subject areas


  • Cell stiffness, Cholesterol, HCC, Ras, V-ATPase