Vacuum microcasting of 2-methacryloyloxyethyl phosphorylcholine polymer for stable cell patterning

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • Nobuyuki Tanaka - , RIKEN Center for Biosystems Dynamics Research (Author)
  • Ryoji Sekine - , RIKEN Center for Biosystems Dynamics Research (Author)
  • Shun-Ichi Funano - , RIKEN Center for Biosystems Dynamics Research (Author)
  • Asako Sato - , RIKEN Center for Biosystems Dynamics Research (Author)
  • Núria Taberner Carretero - , European Molecular Biology Laboratory (EMBL) Barcelona (Author)
  • Miki Ebisuya - , RIKEN Center for Biosystems Dynamics Research, European Molecular Biology Laboratory (EMBL) Barcelona (Author)
  • Yo Tanaka - , RIKEN Center for Biosystems Dynamics Research (Author)

Abstract

This study demonstrates the rapid fabrication and utility of 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer film for cell patterning. The film was obtained on a cell culture surface by microcasting MPC polymer ethanol solution into a degassed polydimethylsiloxane mold with a desired pattern. After removal of the mold, 293AD cells were cultured on the surface of the polymer film with the patterned microstructures. Patterned cell adhesion restricted by the film was successfully maintained during at least a 168-h cultivation. The microcast MPC polymer film can be prepared rapidly and used for efficient long-term cell confinement.

Details

Original languageEnglish
Pages (from-to)171-177
Number of pages7
JournalBioTechniques : the international journal of life science methods
Volume69
Issue number3
Publication statusPublished - Sept 2020
Peer-reviewedYes
Externally publishedYes

External IDs

Scopus 85091126202

Keywords

Keywords

  • Cell Adhesion/drug effects, Dimethylpolysiloxanes/chemistry, Humans, Methacrylates/chemistry, Phosphorylcholine/analogs & derivatives, Polymers/chemistry, Surface Properties, Vacuum