Two Auxinic Herbicides Affect Brassica napus Plant Hormone Levels and Induce Molecular Changes in Transcription

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • Jutta Ludwig-Müller - , Institute of Botany, TUD Dresden University of Technology (Author)
  • Roman Rattunde - , Institut für Botanik (Author)
  • Sabine Rößler - , Institut für Botanik (Author)
  • Katja Liedel - , Institute of Botany, TUD Dresden University of Technology (Author)
  • Freia Benade - , Institut für Botanik (Author)
  • Agnes Rost - , Corteva Agriscience Germany GmbH (Author)
  • Jörg Becker - , Corteva Agriscience Germany GmbH (Author)

Abstract

With the introduction of the new auxinic herbicide halauxifen-methyl into the oilseed rape (Brassica napus) market, there is a need to understand how this new molecule interacts with indigenous plant hormones (e.g., IAA) in terms of crop response. The aim of this study was to investigate the molecular background by using different growth conditions under which three different auxinic herbicides were administered. These were halauxifen-methyl (Hal), alone and together with aminopyralid (AP) as well as picloram (Pic). Three different hormone classes were determined, free and conjugated indole-3-acetic acid (IAA), aminocyclopropane carboxylic acid (ACC) as a precursor for ethylene, and abscisic acid (ABA) at two different temperatures and growth stages as well as over time (2-168 h after treatment). At 15 °C growth temperature, the effect was more pronounced than at 9 °C, and generally, the younger leaves independent of the developmental stage showed a larger effect on the alterations of hormones. IAA and ACC showed reproducible alterations after auxinic herbicide treatments over time, while ABA did not. Finally, a transcriptome analysis after treatment with two auxinic herbicides, Hal and Pic, showed different expression patterns. Hal treatment leads to the upregulation of auxin and hormone responses at 48 h and 96 h. Pic treatment induced the hormone/auxin response already after 2 h, and this continued for the other time points. The more detailed analysis of the auxin response in the datasets indicate a role for GH3 genes and genes encoding auxin efflux proteins. The upregulation of the GH3 genes correlates with the increase in conjugated IAA at the same time points and treatments. Also, genes for were found that confirm the upregulation of the ethylene pathway.

Details

Original languageEnglish
Article number1153
JournalBiomolecules
Volume11
Issue number8
Publication statusPublished - 4 Aug 2021
Peer-reviewedYes

External IDs

PubMedCentral PMC8391463
Scopus 85111745173

Keywords

Keywords

  • Abscisic Acid/metabolism, Amino Acids, Cyclic/metabolism, Brassica napus/drug effects, Carboxylic Acids/pharmacology, Gene Expression Profiling, Gene Expression Regulation, Plant, Gene Ontology, Herbicides/pharmacology, Indoleacetic Acids/metabolism, Molecular Sequence Annotation, Picloram/pharmacology, Plant Growth Regulators/metabolism, Plant Leaves/drug effects, Plant Proteins/genetics, Pyridines/pharmacology, Temperature, Transcriptome

Library keywords