Twenty-seven tamoxifen-inducible icre-driver mouse strains for eye and brain, including seventeen carrying a new inducible-first constitutive-ready allele

Research output: Contribution to journalResearch articleContributedpeer-review


  • Andrea J. Korecki - (Author)
  • Jack W. Hickmott - (Author)
  • Siu Ling Lam - (Author)
  • Lisa Dreolini - (Author)
  • Anthony Mathelier - (Author)
  • Oliver Baker - , Biomedical Genomics (Research Group) (Author)
  • Claudia Kuehne - (Author)
  • Russell J. Bonaguro - (Author)
  • Jillian Smith - (Author)
  • Chin Vern Tan - (Author)
  • Michelle Zhou - (Author)
  • Daniel Goldowitz - (Author)
  • Jan M. Deussing - (Author)
  • A. Francis Stewart - , Chair of Applied Genomics (Author)
  • Wyeth W. Wasserman - (Author)
  • Robert A. Holt - (Author)
  • Elizabeth M. Simpson - (Author)


To understand gene function, the cre/loxP conditional system is the most powerful available for temporal and spatial control of expression in mouse. However, the research community requires more cre recombinase expressing transgenic mouse strains (cre-drivers) that restrict expression to specific cell types. To address these problems, a high-throughput method for large-scale production that produces high-quality results is necessary. Further, endogenous promoters need to be chosen that drive cell type specific expression, or we need to further focus the expression by manipulating the promoter. Here we test the suitability of using knock-ins at the docking site 59 of Hprt for rapid development of numerous cre-driver strains focused on expression in adulthood, using an improved cre tamoxifen inducible allele (icre/ERT2), and testing a novel inducible-first, constitutive-ready allele (icre/f3/ERT2/f3). In addition, we test two types of promoters either to capture an endogenous expression pattern (MaxiPromoters), or to restrict expression further using minimal promoter element(s) designed for expression in restricted cell types (MiniPromoters). We provide new cre-driver mouse strains with applicability for brain and eye research. In addition, we demonstrate the feasibility and applicability of using the locus 59 of Hprt for the rapid generation of substantial numbers of cre-driver strains. We also provide a new inducible-first constitutiveready allele to further speed cre-driver generation. Finally, all these strains are available to the research community through The Jackson Laboratory.


Original languageEnglish
Pages (from-to)1155-1177
Number of pages23
Issue number4
Publication statusPublished - Apr 2019

External IDs

PubMed 30765420
ORCID /0000-0002-4754-1707/work/142248110


ASJC Scopus subject areas


  • Bacterial artificial chromosome, Brain, Cornea, Gene/expression, Hprt locus, Inducible/constitutive, Promoter, Retina, Targeted mutation, Transgenic mice