Transduction of human glial and neuronal tumor cells with different lentivirus vector pseudotypes
Research output: Contribution to journal › Research article › Contributed › peer-review
Contributors
Abstract
Lentiviral vectors have proven to be valuable tools for in vitro and in vivo gene delivery because they can transduce dividing and non-dividing cells efficiently, and mediate long-term gene expression. Pseudotyping of lentiviral vectors with envelope proteins other than VSV-G has resulted in enhanced transduction of certain cell types and tissues. In order to improve lentiviral vector-based gene therapy for peripheral neuroectodermal and brain tumors, we compared the efficiency of eight different lentivirus pseudotypes in transducing neuronal and glial tumor cell lines. Here, lentiviral vectors pseudotyped with the envelopes from human foamy virus, rabies, Mokola or amphotropic murine leukemia virus displayed the highest transduction efficiency in neuroblastomas, whereas pseudotyping with the lymphocytic choriomeningitis virus glycoprotein from strain Armstrong 53b resulted in the highest transduction efficiency in gliomas.
Details
Original language | English |
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Pages (from-to) | 281-8 |
Number of pages | 8 |
Journal | Journal of neuro-oncology |
Volume | 70 |
Issue number | 3 |
Publication status | Published - Dec 2004 |
Peer-reviewed | Yes |
External IDs
ORCID | /0000-0002-0320-4223/work/150885086 |
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Scopus | 11244349670 |
Keywords
Keywords
- Animals, Cell Line, Tumor, Flow Cytometry, Genetic Vectors, Glioma/genetics, Green Fluorescent Proteins/genetics, Humans, Lentivirus/genetics, Neuroblastoma/genetics, Transduction, Genetic/methods