Antibiotic resistance gene (ARG) mobility plays a crucial role in the spread of antimicrobial resistance across One Health settings. Current environmental surveillance often overlooks the significance of ARG mobility, limiting risk assessment accuracy. This perspective highlights that with recent methodological advances in detecting ARG mobility, relevant databases, and improved quantitative microbial risk assessment frameworks, the time to integrate ARG mobility into environmental antimicrobial resistance (AMR) surveillance and risk assessment is now.