Towards a spectrum-based bar code for identification of weakly fluorescent microparticles
Research output: Contribution to journal › Research article › Contributed › peer-review
Spectrally resolved detection of fluorescent probes can be used to identify multiple labeled target molecules in an unknown mixture. We study how the spectral shape, the experimental noise, and the number of spectral detection channels affect the success of identification of weakly fluorescent beads on basis of their emission spectra. The proposed formalism allows to estimate the performance of the spectral identification procedure with a given set of spectral codes on the basis of the reference spectra only. We constructed a simple prismbased setup for spectral detection and demonstrate that seven distinct but overlapping spectral codes realized by combining up to three fluorescent dyes bound to a single bead in a barcodebased manner can be reliably identified. The procedure allows correct identification even in the presence of known autofluorescence background stronger than the actual signal.
|Journal||Methods and applications in fluorescence / Institute of Physics|
|Publication status||Published - Mar 2014|
ASJC Scopus subject areas
- Fluorescence, Multiplexing, Spectral barcoding, Spectral identification