Towards a spectrum-based bar code for identification of weakly fluorescent microparticles

Research output: Contribution to journalResearch articleContributedpeer-review


  • Zdeněk Petrášek - , TUD Dresden University of Technology (Author)
  • Jens Wiedemann - , TUD Dresden University of Technology (Author)
  • Petra Schwille - , Chair of Biophysics (Author)


Spectrally resolved detection of fluorescent probes can be used to identify multiple labeled target molecules in an unknown mixture. We study how the spectral shape, the experimental noise, and the number of spectral detection channels affect the success of identification of weakly fluorescent beads on basis of their emission spectra. The proposed formalism allows to estimate the performance of the spectral identification procedure with a given set of spectral codes on the basis of the reference spectra only. We constructed a simple prismbased setup for spectral detection and demonstrate that seven distinct but overlapping spectral codes realized by combining up to three fluorescent dyes bound to a single bead in a barcodebased manner can be reliably identified. The procedure allows correct identification even in the presence of known autofluorescence background stronger than the actual signal.


Original languageEnglish
Article number015004
Journal Methods and applications in fluorescence / Institute of Physics
Issue number1
Publication statusPublished - Mar 2014



  • Fluorescence, Multiplexing, Spectral barcoding, Spectral identification

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