Time to Upgrade: A New OpenSPIM Guide to Build and Operate Advanced OpenSPIM Configurations

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • Johannes Girstmair - , Max Planck Institute of Molecular Cell Biology and Genetics (Author)
  • Hong Kee Moon - , Max Planck Institute of Molecular Cell Biology and Genetics (Author)
  • Charlène Brillard - , Max Planck Institute of Molecular Cell Biology and Genetics (Author)
  • Robert Haase - , Clusters of Excellence PoL: Physics of Life, Max Planck Institute of Molecular Cell Biology and Genetics (Author)
  • Pavel Tomancak - , Max Planck Institute of Molecular Cell Biology and Genetics (Author)

Abstract

OpenSPIM is an Open Access platform for Selective Plane Illumination Microscopy (SPIM) and allows hundreds of laboratories around the world to generate and process light-sheet data in a cost-effective way due to open-source hardware and software. While setting up a basic OpenSPIM configuration can be achieved expeditiously, correctly assembling and operating more complex OpenSPIM configurations can be challenging for routine standard OpenSPIM users. Detailed instructions on how to equip an OpenSPIM with two illumination sides and two detection axes (X-OpenSPIM) are provided, and a solution is also provided on how the temperature can be controlled in the sample chamber. Additionally, it is demonstrated how to operate it by implementing an ArduinoUNO microcontroller and introducing μOpenSPIM, a new software plugin for OpenSPIM, to facilitate image acquisition. The new software works on any OpenSPIM configuration comes with drift correction functionality, on-the-fly image processing, and gives users more options in the way time-lapse movies are initially set up and saved. Step-by-step guides are also provided within the Supporting Information and on the website on how to align the lasers, configure the hardware, and acquire images using μOpenSPIM. With this, current OpenSPIM users are empowered in various ways, and newcomers striving to use more advanced OpenSPIM systems are helped.

Details

Original languageEnglish
Article number2101182
JournalAdvanced Biology
Volume6
Issue number4
Publication statusPublished - Apr 2022
Peer-reviewedYes

External IDs

PubMed 34761567

Keywords

Keywords

  • CLIJ, light-sheet microscopy, micro-Manager, open sources, OpenSPIM, plugins, softwares, µOpenSPIM

Library keywords