Three-dimensional correlative single-cell imaging utilizing fluorescence and refractive index tomography
Research output: Contribution to journal › Research article › Contributed › peer-review
Contributors
Abstract
Cells alter the path of light, a fact that leads to well-known aberrations in single cell or tissue imaging. Optical diffraction tomography (ODT) measures the biophysical property that causes these aberrations, the refractive index (RI). ODT is complementary to fluorescence imaging and does not require any markers. The present study introduces RI and fluorescence tomography with optofluidic rotation (RAFTOR) of suspended cells, facilitating the segmentation of the 3D-correlated RI and fluorescence data for a quantitative interpretation of the nuclear RI. The technique is validated with cell phantoms and used to confirm a lower nuclear RI for HL60 cells. Furthermore, the nuclear inversion of adult mouse photoreceptor cells is observed in the RI distribution. The applications shown confirm predictions of previous studies and illustrate the potential of RAFTOR to improve our understanding of cells and tissues.
Details
Original language | English |
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Article number | e201700145 |
Journal | Journal of biophotonics |
Volume | 11 |
Issue number | 3 |
Publication status | Published - Mar 2018 |
Peer-reviewed | Yes |
External IDs
PubMed | 28800386 |
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Keywords
ASJC Scopus subject areas
Keywords
- cell nucleus, flow cytometry, optical diffraction tomography, optical trapping, refractive index, retina cells, single-cell analysis, tomography