The relationship between metastatic potential and in vitro mechanical properties of osteosarcoma cells

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • Claude N. Holenstein - (Author)
  • Aron Horvath - (Author)
  • Barbara Schär - (Author)
  • Angelina D. Schoenenberger - (Author)
  • Maja Bollhalder - (Author)
  • Nils Goedecke - (Author)
  • Guido Bartalena - (Author)
  • Oliver Otto - , TUD Dresden University of Technology (Author)
  • Maik Herbig - , TUD Dresden University of Technology (Author)
  • Jochen Guck - , TUD Dresden University of Technology (Author)
  • Daniel A. Müller - (Author)
  • Jess G. Snedeker - (Author)
  • Unai Silvan - (Author)

Abstract

Osteosarcoma is the most frequent primary tumor of bone and is characterized by its high tendency to metastasize in lungs. Although treatment in cases of early diagnosis results in a 5-yr survival rate of nearly 60%, the prognosis for patients with secondary lesions at diagnosis is poor, and their 5-yr survival rate remains below 30%. In the present work, we have used a number of analytical methods to investigate the impact of increased metastatic potential on the biophysical properties and force generation of osteosarcoma cells. With that aim, we used two paired osteosarcoma cell lines, with each one comprising a parental line with low metastatic potential and its experimentally selected, highly metastatic form. Mechanical characterization was performed by means of atomic force microscopy, tensile biaxial deformation, and real-time deformability, and cell traction was measured using two-dimensional and micropost-based traction force microscopy. Our results reveal that the low metastatic osteosarcoma cells display larger spreading sizes and generate higher forces than the experimentally selected, highly malignant variants. In turn, the outcome of cell stiffness measurements strongly depends on the method used and the state of the probed cell, indicating that only a set of phenotyping methods provides the full picture of cell mechanics.

Details

Original languageEnglish
Pages (from-to)887-898
Number of pages12
JournalMolecular Biology of the Cell
Volume30
Issue number7
Publication statusPublished - 2019
Peer-reviewedYes
Externally publishedYes

External IDs

PubMed 30785850

Keywords

ASJC Scopus subject areas