The receptor kinase impaired oomycete susceptibility1 a ttenuates abscisic acid responses in Arabidopsis

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • Sophie Hok - , Institut Sophia Agrobiotech - (ISA) (Author)
  • Valérie Allasia - , Institut Sophia Agrobiotech - (ISA) (Author)
  • Emilie Andrio - , Institut Sophia Agrobiotech - (ISA), INSERM - Institut national de la santé et de la recherche médicale (Author)
  • Elodie Naessens - , Institut Sophia Agrobiotech - (ISA) (Author)
  • Elsa Ribes - , Institut Sophia Agrobiotech - (ISA) (Author)
  • Franck Panabières - , Institut Sophia Agrobiotech - (ISA) (Author)
  • Agnès Attard - , Institut Sophia Agrobiotech - (ISA) (Author)
  • Nicolas Ris - , Institut Sophia Agrobiotech - (ISA) (Author)
  • Mathilde Clément - , Aix-Marseille Université, Institut Sophia Agrobiotech - (ISA) (Author)
  • Xavier Barlet - , INRAE - National Institute of Agricultural Research (Author)
  • Yves Marco - , INRAE - National Institute of Agricultural Research (Author)
  • Erwin Grill - , Technical University of Munich (Author)
  • Ruth Eichmann - , Technical University of Munich, University of Warwick (Author)
  • Corina Weis - , Technical University of Munich (Author)
  • Ralph Hückelhoven - , Technical University of Munich (Author)
  • Alexandra Ammon - , Friedrich-Alexander University Erlangen-Nürnberg (Author)
  • Jutta Ludwig-Müller - , Institute of Botany, Technische Universität Dresden (Author)
  • Lars M. Voll - , Friedrich-Alexander University Erlangen-Nürnberg (Author)
  • Harald Keller - , Institut Sophia Agrobiotech - (ISA) (Author)

Abstract

In plants, membrane-bound receptor kinases are essential for developmental processes, immune responses to pathogens and the establishment of symbiosis. We previously identified the Arabidopsis (Arabidopsis thaliana) receptor kinase IMPAIRED OOMYCETE SUSCEPTIBILITY1 (IOS1) as required for successful infection with the downy mildew pathogen Hyaloperonospora arabidopsidis. We report here that IOS1 is also required for full susceptibility of Arabidopsis to unrelated (hemi)biotrophic filamentous oomycete and fungal pathogens. Impaired susceptibility in the absence of IOS1 appeared to be independent of plant defense mechanism. Instead, we found that ios1-1 plants were hypersensitive to the plant hormone abscisic acid (ABA), displaying enhanced ABA-mediated inhibition of seed germination, root elongation, and stomatal opening. These findings suggest that IOS1 negatively regulates ABA signaling in Arabidopsis. The expression of ABA-sensitive COLD REGULATED and RESISTANCE TO DESICCATION genes was diminished in Arabidopsis during infection. This effect on ABA signaling was alleviated in the ios1-1 mutant background. Accordingly, ABA-insensitive and ABA-hypersensitive mutants were more susceptible and resistant to oomycete infection, respectively, showing that the intensity of ABA signaling affects the outcome of downy mildew disease. Taken together, our findings suggest that filamentous (hemi)biotrophs attenuate ABA signaling in Arabidopsis during the infection process and that IOS1 participates in this pathogen-mediated reprogramming of the host.

Details

Original languageEnglish
Pages (from-to)1506-1518
Number of pages13
JournalPlant physiology
Volume166
Issue number3
Publication statusPublished - 1 Nov 2014
Peer-reviewedYes

External IDs

PubMed 25274985

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