The junctional adhesion molecule-C promotes neutrophil transendothelial migration in vitro and in vivo
Research output: Contribution to journal › Research article › Contributed › peer-review
Contributors
Abstract
The third member of the family of junctional adhesion molecules (JAMs), JAM-3, also called JAM-C, was recently shown to be a novel counter-receptor on platelets for the leukocyte beta(2)-integrin Mac-1 (alphaMbeta(2), CD11b/CD18). Here, new functional aspects of the role of endothelial cell JAM-C were investigated. Endothelial cells express JAM-C, which is predominantly localized within junctions at interendothelial contacts, since it codistributes with a tight junction component, zonula occludens-1. Whereas JAM-C does not participate in neutrophil adhesion to endothelial cells, it mediates neutrophil transmigration in a Mac-1-dependent manner. In particular, inhibition of JAM-C significantly reduced neutrophil transendothelial migration, and the combination of JAM-C and platelet/endothelial cell adhesion molecule-1 blockade almost completely abolished neutrophil transendothelial migration in vitro. In vivo, inhibition of JAM-C with soluble mouse JAM-C resulted in a 50% reduction of neutrophil emigration in the mouse model of acute thioglycollate-induced peritonitis. Thus, JAM-C participates in neutrophil transmigration and thereby provides a novel molecular target for antagonizing interactions between vascular cells that promote inflammatory vascular pathologies.
Details
Original language | English |
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Pages (from-to) | 55602-55608 |
Number of pages | 7 |
Journal | The Journal of biological chemistry |
Volume | 279 |
Issue number | 53 |
Publication status | Published - 31 Dec 2004 |
Peer-reviewed | Yes |
Externally published | Yes |
External IDs
Scopus | 11144304343 |
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Keywords
Sustainable Development Goals
Keywords
- Animals, Blotting, Western, Cell Adhesion, Cell Adhesion Molecules/chemistry, Cell Movement, Cells, Cultured, Endothelial Cells/metabolism, Endothelium, Vascular/cytology, Flow Cytometry, Humans, Immunoblotting, Immunoglobulins/chemistry, In Vitro Techniques, Inflammation, Junctional Adhesion Molecules, Membrane Proteins/chemistry, Mice, Microscopy, Fluorescence, Neutrophils/cytology, Peritonitis/pathology, Phosphoproteins/metabolism, Protein Binding, Tetradecanoylphorbol Acetate/pharmacology, Zonula Occludens-1 Protein