The expression pattern and assembly profile of synaptic membrane proteins in ribbon synapses of the developing mouse retina

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

Abstract

In the present study, we generated a systematic overview of the expression pattern and assembly profile of synaptic membrane proteins in ribbon synapses of the developing mouse retina. Using indirect immunofluorescence microscopy, we analyzed the spatial and temporal distribution of 11 important membrane and membrane-associated synaptic proteins (syntaxin 1/3, SNAP-25, synaptobrevin 2, synaptogyrin, synaptotagmin I, SV2A, SV2B, Rab3A, clathrin light chains, CSP and neuroligin I) during synaptogenesis. The temporospatial distribution of these synaptic proteins was "normalized" by the simultaneous visualization of the synaptic vesicle protein synaptophysin, which served as an internal reference protein. We found that expression of various synaptic membrane proteins started at different time points and changed progressively during development. At early stages of development synaptic vesicle membrane proteins at extrasynaptic locations did not always colocalize with synaptophysin, indicating that these proteins probably do not reside in the same transport vesicles. Despite a non-synchronized onset of protein expression, clustering and colocalization of all synaptic membrane proteins at ribbon synapses roughly occurred in the same time window (between day 4 after birth, P4, and P5). Thus, the basic synaptic membrane machinery is already present in ribbon synapses before the well-known complete morphological maturation of ribbon synapses between P7 and P12. We conclude that ribbon synapse formation is a multistep process in which the concerted recruitment of synaptic membrane proteins is a relatively early event and clearly not the final step.

Details

Original languageEnglish
Pages (from-to)159-73
Number of pages15
JournalCell and tissue research
Volume311
Issue number2
Publication statusPublished - Feb 2003
Peer-reviewedYes
Externally publishedYes

External IDs

Scopus 0037294080
ORCID /0000-0001-7989-5860/work/147142864

Keywords

Keywords

  • Aging/physiology, Animals, Clathrin/genetics, Fluorescent Antibody Technique, Indirect, Gene Expression Regulation, Developmental, Membrane Proteins/genetics, Mice, Mice, Inbred Strains, Nerve Tissue Proteins/genetics, Retina/growth & development, Synapses/physiology, Synaptosomal-Associated Protein 25, rab3A GTP-Binding Protein/genetics