The effect of octadecyl chain immobilization on the hemocompatibility of poly (2-hydroxyethyl methacrylate)

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • Marion Fischer - , Leibniz Institute of Polymer Research Dresden (Author)
  • Catarina P. Baptista - , University of Porto (Author)
  • Inês C. Gonçalves - , University of Porto (Author)
  • Buddy D. Ratner - , University of Washington (Author)
  • Claudia Sperling - , Leibniz Institute of Polymer Research Dresden (Author)
  • Carsten Werner - , Chair of Biofunctional Polymer Materials, Max Bergmann Center of Biomaterials Dresden, Leibniz Institute of Polymer Research Dresden (Author)
  • Cristina L. Martins - , University of Porto (Author)
  • Mário A. Barbosa - , University of Porto (Author)

Abstract

Albumin-scavenging surfaces decorated with n-alkyl chains represent an established strategy for blood-contacting applications. To evaluate this concept, a set of poly (2-hydroxyethyl methacrylate) (pHEMA) films modified with different amounts of octadecyl isocyanate (C18) was investigated in an in vitro hemocompatibility assay using freshly drawn human whole blood. In addition, the hydrogel materials were thoroughly characterized with respect to changes in wettability and elasticity, which accompanied the gradual chemical modification of pHEMA. An increase of the surface C18 content induced enhanced hydrophobicity and stiffness. Immobilization of C18 chains was found to substantially reduce the coagulation activation and the complement activation by the pHEMA films. Platelet adhesion and degranulation (PF4 release) were similar on the modified and the unmodified pHEMA. Platelet adhesion to pHEMA hydrogels was lower than the polytetrafluoroethylene reference. We conclude that the immobilization of octadecyl chains improved the hemocompatibility of pHEMA materials under conditions that might be encountered in low shear blood flow.

Details

Original languageEnglish
Pages (from-to)7677-7685
Number of pages9
JournalBiomaterials
Volume33
Issue number31
Publication statusPublished - Nov 2012
Peer-reviewedYes

External IDs

PubMed 22840226
ORCID /0000-0003-0189-3448/work/162347629

Keywords

Keywords

  • Albumin, Biomaterials, Haemocompatibility, Platelets, PolyHEMA, Protein adsorption