The calcium sensor CBL7 is required for Serendipita indica-induced growth stimulation in Arabidopsis thaliana, controlling defense against the endophyte and K+ homoeostasis in the symbiosis

Research output: Contribution to journalResearch articleContributedpeer-review


  • Marta-Marina Pérez-Alonso - , Centro de Biotecnología y Genómica de Plantas (Author)
  • Carmen Guerrero-Galán - , Centro de Biotecnología y Genómica de Plantas (Author)
  • Adrián González Ortega-Villaizán - , Centro de Biotecnología y Genómica de Plantas (Author)
  • Paloma Ortiz-García - , Centro de Biotecnología y Genómica de Plantas (Author)
  • Sandra S Scholz - , Friedrich Schiller University Jena (Author)
  • Patricio Ramos - , Centro de Investigación de Estudios Avanzados del Maule (Author)
  • Hitoshi Sakakibara - , RIKEN Center for Sustainable Resource Science (Author)
  • Takatoshi Kiba - , RIKEN Center for Sustainable Resource Science (Author)
  • Jutta Ludwig-Müller - , Institute of Botany, Chair of Plant Physiology (Author)
  • Anne Krapp - , Université Paris-Saclay (Author)
  • Ralf Oelmüller - , Friedrich Schiller University Jena (Author)
  • Jesús Vicente-Carbajosa - , Centro de Biotecnología y Genómica de Plantas (Author)
  • Stephan Pollmann - , Centro de Biotecnología y Genómica de Plantas (Author)


Calcium is an important second messenger in plants. The activation of Ca 2+ signalling cascades is critical in the activation of adaptive processes in response to environmental stimuli. Root colonization by the growth promoting endophyte Serendipita indica involves the increase of cytosolic Ca 2+ levels in Arabidopsis thaliana. Here, we investigated transcriptional changes in Arabidopsis roots during symbiosis with S. indica. RNA-seq profiling disclosed the induction of Calcineurin B-like 7 (CBL7) during early and later phases of the interaction. Consistently, reverse genetic evidence highlighted the functional relevance of CBL7 and tested the involvement of a CBL7-CBL-interacting protein kinase 13 signalling pathway. The loss-of-function of CBL7 abolished the growth promoting effect and affected root colonization. The transcriptomics analysis of cbl7 revealed the involvement of this Ca 2+ sensor in activating plant defense responses. Furthermore, we report on the contribution of CBL7 to potassium transport in Arabidopsis. We analysed K + contents in wild-type and cbl7 plants and observed a significant increase of K + in roots of cbl7 plants, while shoot tissues demonstrated K + depletion. Taken together, our work associates CBL7 with an important role in the mutual interaction between Arabidopsis and S. indica and links CBL7 to K + transport.


Original languageEnglish
Pages (from-to)3367-3382
Number of pages16
JournalPlant, cell & environment
Issue number11
Early online date19 Aug 2022
Publication statusPublished - Nov 2022

External IDs

Scopus 85136840243
Mendeley c0b0e738-19c8-3df8-973d-54b556e94182


ASJC Scopus subject areas


  • Arabidopsis/metabolism, Arabidopsis Proteins/metabolism, Basidiomycota/metabolism, Calcineurin/genetics, Calcium/metabolism, Endophytes/metabolism, Gene Expression Regulation, Plant, Homeostasis, Plant Roots/metabolism, Plants/metabolism, Potassium/metabolism, Protein Kinases/metabolism, Symbiosis, growth promotion, plant−microbe interaction, Ca signalling, plant defense, endosymbiosis, plant performance, Ca2+ signalling

Library keywords