Targeted drug delivery using genetically engineered diatom biosilica
Research output: Contribution to journal › Research article › Contributed › peer-review
Contributors
Abstract
The ability to selectively kill cancerous cell populations while leaving healthy cells unaffected is a key goal in anticancer therapeutics. The use of nanoporous silica-based materials as drug-delivery vehicles has recently proven successful, yet production of these materials requires costly and toxic chemicals. Here we use diatom microalgae-derived nanoporous biosilica to deliver chemotherapeutic drugs to cancer cells. The diatom Thalassiosira pseudonana is genetically engineered to display an IgG-binding domain of protein G on the biosilica surface, enabling attachment of cell-targeting antibodies. Neuroblastoma and B-lymphoma cells are selectively targeted and killed by biosilica displaying specific antibodies sorbed with drug-loaded nanoparticles. Treatment with the same biosilica leads to tumour growth regression in a subcutaneous mouse xenograft model of neuroblastoma. These data indicate that genetically engineered biosilica frustules may be used as versatile 'backpacks' for the targeted delivery of poorly water-soluble anticancer drugs to tumour sites.
Details
Original language | English |
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Article number | 8791 |
Journal | Nature communications |
Volume | 6 |
Publication status | Published - 10 Nov 2015 |
Peer-reviewed | Yes |
External IDs
Scopus | 84946935108 |
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Keywords
Sustainable Development Goals
Keywords
- Animals, Antibodies, Antineoplastic Agents/administration & dosage, Camptothecin/administration & dosage, Cell Line, Tumor, Cloning, Molecular, Diatoms/genetics, Drug Delivery Systems, Gene Expression Regulation, Genetic Engineering, Immunoglobulin G, Liposomes, Lymphoma, B-Cell/drug therapy, Mice, Micelles, Nanoparticles, Neoplasms, Experimental/drug therapy, Neuroblastoma/drug therapy, Particle Size, Recombinant Fusion Proteins/genetics, Silicon Dioxide/metabolism, Transplantation, Heterologous