Targeted 64 Cu-labeled gold nanoparticles for dual imaging with positron emission tomography and optical imaging

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • Marc Pretze - , Heidelberg University  (Author)
  • Nick P van der Meulen - , Center of Radiopharmaceutical Sciences (Author)
  • Carmen Wängler - , Heidelberg University  (Author)
  • Roger Schibli - , Center of Radiopharmaceutical Sciences (Author)
  • Björn Wängler - , Heidelberg University  (Author)

Abstract

Gold nanoparticles (AuNPs) have been used for many years in cancer treatment mainly for brachytherapy, but in the last 15 years, the focus has shifted to the development of ultrasmall target-specific AuNPs with homogeneous size and, ultimately, tailored shapes for use in various imaging modalities such as computed tomography (CT), Raman, or photoacoustic imaging. Here, we report on the development of tumor-specific AuNPs as diagnostic tools intended for the dual detection of prostate cancer via optical imaging (OI) and positron emission tomography (PET). The AuNPs were decorated with a near-infrared dye and NODAGA chelator for complexation with radiometals. Radiolabeling with 64 Cu was performed either indirect by complexation with NODAGA-AuNPs or by direct reduction of [64 Cu]Cu(0) onto the surface of the AuNPs. Both methods yielded stable 64 Cu-AuNPs with radiochemical yield more than 95% confirmed by HPLC. 64 Cu-AuNPs were evaluated in a dual-imaging setting in vitro and in vivo and exhibited favorable diagnostic properties concerning detection, biodistribution, and clearance. Furthermore, the first therapeutic properties of the 64 Cu-AuNPs were evaluated in vitro concerning acute and long-term toxicity, indicating that these 64 Cu-AuNPs could be used in therapeutic concepts in the future.

Details

Original languageEnglish
Pages (from-to)471-482
Number of pages12
JournalJournal of labelled compounds & radiopharmaceuticals
Volume62
Issue number8
Publication statusPublished - 30 Jun 2019
Peer-reviewedYes
Externally publishedYes

External IDs

Scopus 85068898061
ORCID /0000-0002-6432-5694/work/146644235

Keywords

Sustainable Development Goals

Keywords

  • Animals, Copper Radioisotopes/chemistry, Gold/chemistry, Humans, Isotope Labeling, Male, Metal Nanoparticles/chemistry, Mice, Optical Imaging/methods, PC-3 Cells, Positron Emission Tomography Computed Tomography/methods, Tissue Distribution

Library keywords