SWAP-70-deficient mast cells are impaired in development and IgE-mediated degranulation
Research output: Contribution to journal › Research article › Contributed › peer-review
Contributors
Abstract
Cross-linking of the high-affinity IgE receptor (FcepsilonRI) on mast cell activates signaling pathways that trigger degranulation and the release of multiple pro-inflammatory mediators. Mature,immature and precursor mast cells are degranulation competent. We show here that the signaling protein SWAP-70 has a function in mast cell biology. While not found in many cell types, we find that apart from B cells, mast cells also express SWAP-70. In activated B cells, SWAP-70 shuttles between cytoplasm and nucleus, but in mast cells it is confined to the cytoplasm. SWAP-70(ko/ko) (double knockout) mice have reduced numbers of mature mast cells, and these are degranulation competent. However, although immature mast cells from SWAP-70(ko/ko) mice respond normally to SCF and IL-3 and have functional granules, their FcepsilonRI-mediated degranulation is inhibited. Thus, in mast cells SWAP-70 plays a role both in establishing the initial competence to degranulate and to develop into mature mast cells.
Details
Original language | English |
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Pages (from-to) | 1121-8 |
Number of pages | 8 |
Journal | European Journal of Immunology |
Volume | 32 |
Issue number | 4 |
Publication status | Published - Apr 2002 |
Peer-reviewed | Yes |
External IDs
Scopus | 0036235590 |
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Keywords
Keywords
- Animals, B-Lymphocytes/metabolism, Cell Differentiation, Cytoplasm/metabolism, Cytoplasmic Granules/metabolism, DNA-Binding Proteins/deficiency, Exocytosis/drug effects, Guanine Nucleotide Exchange Factors, Immunoglobulin E/immunology, Interleukin-3/pharmacology, Mast Cells/cytology, Mice, Mice, Knockout, Minor Histocompatibility Antigens, Nuclear Proteins/deficiency, Receptors, IgE/immunology, Signal Transduction, Stem Cell Factor/pharmacology