Surface Functionalization of Poly(L-lactide-co-glycolide) Membranes with RGD-Grafted Poly(2-oxazoline) for Periodontal Tissue Engineering

Research output: Contribution to journalResearch articleContributedpeer-review


  • Anna M. Tryba - , AGH University of Science and Technology (Author)
  • Małgorzata Krok-Borkowicz - , AGH University of Science and Technology (Author)
  • Michał Kula - , AGH University of Science and Technology (Author)
  • Natalia Piergies - , Polish Academy of Sciences (Author)
  • Mateusz Marzec - , AGH University of Science and Technology (Author)
  • Erik Wegener - , Chair of Macromolecular Chemistry (Author)
  • Justyna Frączyk - , Lodz University of Technology (Author)
  • Rainer Jordan - , Chair of Macromolecular Chemistry (Author)
  • Beata Kolesińska - , Lodz University of Technology (Author)
  • Dieter Scharnweber - , Chair of Biomaterials (Author)
  • Czesława Paluszkiewicz - , Polish Academy of Sciences (Author)
  • Elżbieta Pamuła - , AGH University of Science and Technology (Author)


Bone tissue defects resulting from periodontal disease are often treated using guided tissue regeneration (GTR). The barrier membranes utilized here should prevent soft tissue infiltration into the bony defect and simultaneously support bone regeneration. In this study, we designed a degradable poly(L-lactide-co-glycolide) (PLGA) membrane that was surface-modified with cell adhesive arginine-glycine-aspartic acid (RGD) motifs. For a novel method of membrane manufacture, the RGD motifs were coupled with the non-ionic amphiphilic polymer poly(2-oxazoline) (POx). The RGD-containing membranes were then prepared by solvent casting of PLGA, POx coupled with RGD (POx_RGD), and poly(ethylene glycol) (PEG) solution in methylene chloride (DCM), followed by DCM evaporation and PEG leaching. Successful coupling of RGD to POx was confirmed spec-troscopically by Raman, Fourier transform infrared in attenuated reflection mode (FTIR-ATR), and X-ray photoelectron (XPS) spectroscopy, while successful immobilization of POx_RGD on the membrane surface was confirmed by XPS and FTIR-ATR. The resulting membranes had an asymmetric microstructure, as shown by scanning electron microscopy (SEM), where the glass-cured surface was more porous and had a higher surface area then the air-cured surface. The higher porosity should support bone tissue regeneration, while the air-cured side is more suited to preventing soft tissue infiltration. The behavior of osteoblast-like cells on PLGA membranes modified with POx_RGD was compared to cell behavior on PLGA foil, non-modified PLGA membranes, or PLGA membranes modified only with POx. For this, MG-63 cells were cultured for 4, 24, and 96 h on the membranes and analyzed by metabolic activity tests, live/dead staining, and fluorescent staining of actin fibers. The results showed bone cell adhesion, proliferation, and viability to be the highest on membranes modified with POx_RGD, making them possible candidates for GTR applications in periodontology and in bone tissue engineering.


Original languageEnglish
Article number4
JournalJournal of functional biomaterials
Issue number1
Publication statusPublished - Jan 2022

External IDs

Bibtex jfb13010004


ASJC Scopus subject areas


  • Bone tissue engineering, Guided tissue regeneration (GTR), Osteoblast-like cells, Periodontology, Phase separation, Poly(2-oxazoline), Poly(ethylene glycol), Poly(L-lactide-co-glycolide), RGD sequences