Structure and subunit arrangement of the A-type ATP synthase complex from the archaeon Methanococcus jannaschii visualized by electron microscopy
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Contributors
Abstract
In Archaea, bacteria, and eukarya, ATP provides metabolic energy for energy-dependent processes. It is synthesized by enzymes known as A-type or F-type ATP synthase, which are the smallest rotatory engines in mature (Yoshida, M., Muneyuki, E., and Hisabori, T. (2001) Nat Rev. Mol. Cell Biol. 2, 689-677; Imamura, H., Nakano, M., Noji, H., Muneyuki, E., Ohkuma, S., Yoshida, M., and Yokoyama, E. (2003) Proc. Natl. Acad. Sci. U. S. A. 100, 2312-2315). Here, we report the first projected structure of an intact A1A0 ATP synthase from Methanococcus jannaschii as determined by electron microscopy and single particle analysis at a resolution of 1.8 nm. The enzyme with an overall length of 25.9 nm is organized in an A1 headpiece (9.4 x 11.5 nm) and a membrane domain, A0 (6.4 x 10.6 nm), which are linked by a central stalk with a length of ∼8 nm. A part of the central stalk is surrounded by a horizontal-situated rod-like structure ("collar"), which interacts with a peripheral stalk extending from the A0 domain up to the top of the A1 portion, and a second structure connecting the collar structure with A1. Superposition of the three-dimensional reconstruction and the solution structure of the A1 complex from Methanosarcina mazei Gö1 have allowed the projections to be interpreted as the A1 headpiece, a central and the peripheral stalk, and the integral A0 domain. Finally, the structural organization of the A1A0 complex is discussed in tenus of the structural relationship to the related motors, F1F0 ATP synthase and V1V0 ATPases.
Details
Original language | English |
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Pages (from-to) | 38644-38648 |
Number of pages | 5 |
Journal | Journal of Biological Chemistry |
Volume | 279 |
Issue number | 37 |
Publication status | Published - 10 Sept 2004 |
Peer-reviewed | Yes |
Externally published | Yes |
External IDs
WOS | 000223684100072 |
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Scopus | 4644343538 |
PubMed | 15220347 |