Specific immune recognition of pancreatic carcinoma by patient-derived CD4 and CD8 T cells and its improvement by interferon-γ

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • F. H. Schmitz-Winnenthal - , Heidelberg University  (Author)
  • L. V. Galindo Escobedo - , Heidelberg University  (Author)
  • Philipp Beckhove - , German Cancer Research Center (DKFZ) (Author)
  • V. Schirrmacher - , German Cancer Research Center (DKFZ) (Author)
  • M. Bucur - , German Cancer Research Center (DKFZ) (Author)
  • Y. Ziouta - , German Cancer Research Center (DKFZ) (Author)
  • C. Volk - , Heidelberg University  (Author)
  • B. Schmied - , Heidelberg University  (Author)
  • M. Koch - , Heidelberg University  (Author)
  • D. Antolovic - , Heidelberg University  (Author)
  • J. Weitz - , Heidelberg University  (Author)
  • M. W. Büchler - , Heidelberg University  (Author)
  • K. Z'Graggen - , Clinic Beau-Site (Author)

Abstract

Pancreatic carcinoma is a very aggressive disease and little is known about its immunobiology. We here describe the presence in pancreatic cancer patients of spontaneously induced functional CD4 and CD8 memory/effector T cells reactive to autologous tumor cells or to the pancreatic cancer associated antigen, MUC-1. Such specific cells were present in the bone marrow or peripheral blood of most of the 23 tested patients. Low dose stimulation of primary cultures of pancreatic cancer cells with 500 IU/ml IFN-γ for 72 h enhanced HLA-I expression and induced the de novo expression of HLA-II molecules. This led to a much better immune recognition by autologous HLA-I restricted and purified CD8 T cells and allowed tumor cell recognition by HLA-II restricted purified CD4 T-helper cells. Thus, interferon-γ appears to be a useful adjuvant cytokine to enhance the immunogenicity of a patients' tumor cells and their recognition by tumor reactive immune cells.

Details

Original languageEnglish
Pages (from-to)1419-1428
Number of pages10
JournalInternational journal of oncology
Volume28
Issue number6
Publication statusPublished - Jun 2006
Peer-reviewedYes
Externally publishedYes

External IDs

PubMed 16685444

Keywords

Sustainable Development Goals

ASJC Scopus subject areas

Keywords

  • Antigen presentation, Immunotherapy, Interferon-γ, Pancreatic cancer, Tumor-specific T cells