Spatially resolved elemental distributions in articular cartilage
Research output: Contribution to journal › Conference article › Contributed › peer-review
Contributors
Abstract
In this study, the nuclear microprobe technique is employed to analyse the chemistry of joint cartilage in order to correlate internal structures of the collagen network with the elemental distribution. The samples were taken from pig's knee joint. 30 μm thick coronar cross-sections were prepared by means of cryosectioning and freeze-drying. We performed simultaneously particle induced X-ray emission (PIXE), Rutherford backscattering spectrometry (RBS) and elastic recoil detection analysis (ERDA). Thus we obtained spatially resolved distributions of the elements H, C, N, O, P, S, Cl, K and Ca. The main components of the organic matrix are H, C, N and O. It was shown that their relations vary with the cartilage structures. It could be shown that zones with aligned collagen fibrils contain less sulphur and potassium but more chlorine. The higher chlorine concentration is remarkable because newest biochemical studies found that hypochloric acid is involved in cartilage degradation. Furthermore, the calci um distribution is still of great interest. Its correlation to structural changes inside the cartilage is still being discussed. It could be disproved that zones of higher calcium concentration are related to the aligned structures of the collagen network.
Details
Original language | English |
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Pages (from-to) | 516-521 |
Number of pages | 6 |
Journal | Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms |
Volume | 181 |
Issue number | 1-4 |
Publication status | Published - Jul 2001 |
Peer-reviewed | Yes |
Externally published | Yes |
Conference
Title | 7th International Conference on Nuclear Microprobe Technology (ICNMTA-2000) |
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Duration | 10 - 15 September 2000 |
City | Bordeaux |
Country | France |
External IDs
ORCID | /0000-0001-8204-5699/work/154738452 |
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Keywords
ASJC Scopus subject areas
Keywords
- Arthrosis, Articular cartilage, Elemental analysis, Hydrogen analysis, Micro-PIXE