Solid-phase extraction and high-performance liquid chromatographic determination of articaine and its metabolite articainic acid in human serum
Research output: Contribution to journal › Research article › Contributed › peer-review
Contributors
Abstract
A new method is described using solid-phase extraction (SPE) for preconcentration of articaine and the metabolite articainic acid and high-performance liquid chromatography (HPLC) for the determination of both compounds in human serum. Articaine and articainic acid were extracted in one step with SDB-RPS disk cartridges after precipitation of the serum proteins by perchloric acid. The HPLC separation was then performed on a reversed-phase C8 column using phosphate buffer-acetonitrile (88:12, v/v). UV absorption at 274 nm was used for measuring the analytes with a low limit of quantitation of about 10 ng/ml, which is appropriate for pharmacokinetic studies of low dose submucosal injections of the local anaesthetic agent articaine hydrochloride in dentistry. Copyright (C) 1999 Elsevier Science B.V.
Details
Original language | English |
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Pages (from-to) | 109-115 |
Number of pages | 7 |
Journal | Journal of Chromatography B |
Volume | 724 |
Issue number | 1 |
Publication status | Published - 5 Mar 1999 |
Peer-reviewed | Yes |
External IDs
PubMed | 10202963 |
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ORCID | /0000-0003-1526-997X/work/142247292 |
Keywords
ASJC Scopus subject areas
Keywords
- Articaine, Articainic acid