Sleep-active neuron specification and sleep induction require FLP-11 neuropeptides to systemically induce sleep
Research output: Contribution to journal › Research article › Contributed › peer-review
Contributors
Abstract
Sleep is an essential behavioral state. It is induced by conserved sleep-active neurons that express GABA. However, little is known about how sleep neuron function is determined and how sleep neurons change physiology and behavior systemically. Here, we investigated sleep in Caenorhabditis elegans, which is induced by the single sleep-active neuron RIS. We found that the transcription factor LIM-6, which specifies GABAergic function, in parallel determines sleep neuron function through the expression of APTF-1, which specifies the expression of FLP-11 neuropeptides. Surprisingly FLP-11, and not GABA, is the major component that determines the sleep-promoting function of RIS. FLP-11 is constantly expressed in RIS. At sleep onset RIS depolarizes and releases FLP-11 to induce a systemic sleep state.
Details
Original language | English |
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Article number | e12499 |
Journal | eLife |
Volume | 5 |
Publication status | Published - 7 Mar 2016 |
Peer-reviewed | Yes |
External IDs
PubMedCentral | PMC4805538 |
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Scopus | 84964296807 |
ORCID | /0000-0002-7689-8617/work/142236985 |
Keywords
Keywords
- Animals, Caenorhabditis elegans/physiology, Caenorhabditis elegans Proteins/metabolism, GABAergic Neurons/physiology, LIM-Homeodomain Proteins/metabolism, Neuropeptides/metabolism, Sleep, Thiolester Hydrolases/metabolism, Transcription Factors/metabolism