Single-molecule approaches reveal outer membrane protein biogenesis dynamics
Research output: Contribution to journal › Research article › Contributed › peer-review
Contributors
Abstract
Outer membrane proteins (OMPs) maintain the viability of Gram-negative bacteria by functioning as receptors, transporters, ion channels, lipases, and porins. Folding and assembly of OMPs involves synchronized action of chaperones and multi-protein machineries which escort the highly hydrophobic polypeptides to their target outer membrane in a folding competent state. Previous studies have identified proteins and their involvement along the OMP biogenesis pathway. Yet, the mechanisms of action and the intriguing ability of all these molecular machines to work without the typical cellular energy source of ATP, but solely based on thermodynamic principles, are still not well understood. Here, we highlight how different single-molecule studies can shed additional light on the mechanisms and kinetics of OMP biogenesis.
Details
Original language | English |
---|---|
Article number | 2200149 |
Journal | BIOESSAYS |
Volume | 44 |
Issue number | 12 |
Early online date | 25 Oct 2022 |
Publication status | Published - Dec 2022 |
Peer-reviewed | Yes |
External IDs
Scopus | 85140382765 |
---|---|
Mendeley | dc21d082-2f12-37e3-b719-5f707d91d499 |
ORCID | /0000-0002-6209-2364/work/142237701 |
ORCID | /0000-0002-4657-9092/work/142247777 |
Keywords
ASJC Scopus subject areas
Keywords
- AFM, BAM, FCS, OMP biogenesis, SecYEG, Skp, SurA, dynamics, single-molecule FRET