Simultaneous determination of bupivacaine, mepivacain, prilocaine and ropivacain in human serum by liquid chromatography-tandem mass spectrometry

Research output: Contribution to journalResearch articleContributedpeer-review



A liquid chromatography-tandem mass spectrometric (LC-MS-MS) method with a rapid and simple sample preparation was developed and validated for the simultaneous determination of the local anesthetics bupivacaine, mepivacaine, prilocaine and ropivacaine in human serum. An external calibration was used. The mass spectrometer was operated in the multiple reaction monitoring mode. A good quadratic response over the range of 1.0-200.0 ng/ml was demonstrated. The accuracy for bupivacaine ranged from 93.2 to 105.7%, for mepivacaine from 96.2 to 104.3%, for prilocaine from 94.6 to 105.7% and for ropivacaine from 94.3 to 104.0%, respectively. The limit of quantification was 1.0 ng/ml for all substances. This method is suitable for pharmacokinetic studies.


Original languageEnglish
Pages (from-to)126-130
Number of pages5
JournalJournal of chromatography : including electrophoresis, mass spectrometry and other separation and detection methods : A
Issue number1-2
Publication statusPublished - 23 Sept 2005

External IDs

Scopus 23044439502
PubMed 16130740
ORCID /0000-0003-1526-997X/work/142247208



  • Bupivacaine, LC-MS-MS, Mepivacaine, Prilocaine, Ropivacaine