Sensitive method for the determination of the antiarrhythmic drug detajmium in serum by solid-phase extraction and high-performance liquid chromatography with fluorimetric detection

Research output: Contribution to journalResearch articleContributedpeer-review



The objective of this study was to develop a very sensitive and selective method for the determination of detajmium (4-3-diethylamino-2-hydroxypropyl-ajmaline), a sodium-channel-blocking drug with antiarrhythmic properties, in serum. A high-performance liquid chromatography (HPLC) method with solid-phase extraction and fluorimetric detection has been applied. Serum samples were diluted with phosphate buffer (pH 3.5) and the extraction of detajmium and ajmaline, which was used as an internal standard, was carried out with Oasis cartridges (Waters). The chromatographic separation was performed on a RP18 column. The limit of quantification for serum samples of detajmium was 1 ng/ml with good reproducibility (R.S.D. < 15%) and a linear response from 1 to 200 ng/ml. The described method is highly sensitive and specific for the determination of detajmium in serum of patients and volunteers.


Original languageEnglish
Pages (from-to)217-222
Number of pages6
JournalJournal of chromatography : including electrophoresis, mass spectrometry and other separation and detection methods : A
Issue number1-2
Publication statusPublished - 18 Jun 1999

External IDs

PubMed 10420613
ORCID /0000-0003-1526-997X/work/142247276



  • Ajmaline, Detajmium