RNA-Induced Conformational Switching and Clustering of G3BP Drive Stress Granule Assembly by Condensation

Research output: Contribution to journal › Research article › Contributed › peer-review

Contributors

  • Jordina Guillén-Boixet - , Chair of Cellular Biochemistry (Author)
  • Andrii Kopach - , Max Planck Institute of Molecular Cell Biology and Genetics (Author)
  • Alex S. Holehouse - , Washington University St. Louis (Author)
  • Sina Wittmann - , Max Planck Institute of Molecular Cell Biology and Genetics (Author)
  • Marcus Jahnel - , Chair of Biophysics (Author)
  • Raimund Schlüßler - , Chair of Cellular Biochemistry (Author)
  • Kyoohyun Kim - , Chair of Cellular Machines (Author)
  • Irmela R.E.A. Trussina - , Chair of Cellular Biochemistry (Author)
  • Jie Wang - , Max Planck Institute of Molecular Cell Biology and Genetics (Author)
  • Daniel Mateju - , Max Planck Institute of Molecular Cell Biology and Genetics (Author)
  • Ina Poser - , Max Planck Institute of Molecular Cell Biology and Genetics (Author)
  • Shovamayee Maharana - , Chair of Cellular Biochemistry (Author)
  • Martine Ruer-Gruß - , Max Planck Institute of Molecular Cell Biology and Genetics (Author)
  • Doris Richter - , TUD Dresden University of Technology (Author)
  • Xiaojie Zhang - , European Molecular Biology Laboratory (EMBL) Heidelberg (Author)
  • Young Tae Chang - , Institute for Basic Science, Pohang University of Science and Technology (Author)
  • Jochen Guck - , Chair of Cellular Machines (Author)
  • Alf Honigmann - , Max Planck Institute of Molecular Cell Biology and Genetics (Author)
  • Julia Mahamid - , European Molecular Biology Laboratory (EMBL) Heidelberg (Author)
  • Anthony A. Hyman - , Max Planck Institute of Molecular Cell Biology and Genetics (Author)
  • Rohit V. Pappu - , Washington University St. Louis (Author)
  • Simon Alberti - , Chair of Cellular Biochemistry (Author)
  • Titus M. Franzmann - , Chair of Cellular Biochemistry (Author)

Abstract

Reconstitution of stress granule assembly reveals an autoinhibitory conformation of G3BP that is alleviated by RNA binding, demonstrating how this central node of the stress granule network phase-separates in response to rising cellular RNA concentrations.

Details

Original languageEnglish
Pages (from-to)346-361.e17
JournalCell
Volume181
Issue number2
Publication statusPublished - 16 Apr 2020
Peer-reviewedYes

External IDs

PubMed 32302572
ORCID /0000-0003-4017-6505/work/142253853
ORCID /0000-0003-0475-3790/work/155291285

Keywords

ASJC Scopus subject areas

Keywords

  • G3BP, liquid-to-solid transition, Neurodegenerative disease, phase separation, RNP granules, stress granules, stress response

Library keywords