RhoGDIβ affects HeLa cell spindle orientation following UVC irradiation
Research output: Contribution to journal › Research article › Contributed › peer-review
Contributors
Abstract
The molecular signals that regulate mitotic spindle orientation to determine the proper division axis play a critical role in the development and maintenance of tissue homeostasis. However, deregulation of signaling events can result in spindle misorientation, which in turn can trigger developmental defects and cancer progression. Little is known about the cellular signaling pathway involved in the misorientation of proliferating cells that evade apoptosis after DNA damage. In this study, we found that perturbations to spindle orientation were induced in ultraviolet C (UVC)-irradiated surviving cells. N-terminal truncated Rho GDP-dissociation inhibitor β (RhoGDIβ), which is produced by UVC irradiation, distorted the spindle orientation of HeLa cells cultured on Matrigel. The short hairpin RNA-mediated knockdown of RhoGDIβ significantly attenuated UVC-induced misorientation. Subsequent expression of wild-type RhoGDIβ, but not a noncleavable mutant, RhoGDIβ (D19A), again led to a relative increase in spindle misorientation in response to UVC. Our findings revealed that RhoGDIβ impacts spindle orientation in response to DNA damage.
Details
Original language | English |
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Pages (from-to) | 15134-15146 |
Number of pages | 13 |
Journal | Journal of cellular physiology |
Volume | 234 |
Issue number | 9 |
Publication status | Published - Sept 2019 |
Peer-reviewed | Yes |
External IDs
Scopus | 85060169659 |
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