Rheology of the Active Cell Cortex in Mitosis

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • Elisabeth Fischer-Friedrich - , Max Planck Institute of Molecular Cell Biology and Genetics, Max-Planck-Institute for the Physics of Complex Systems (Author)
  • Yusuke Toyoda - , Kurume University (Author)
  • Cedric J Cattin - , ETH Zurich (Author)
  • Daniel J Müller - , ETH Zurich (Author)
  • Anthony A Hyman - , Max Planck Institute of Molecular Cell Biology and Genetics (Author)
  • Frank Jülicher - , Max-Planck-Institute for the Physics of Complex Systems (Author)

Abstract

The cell cortex is a key structure for the regulation of cell shape and tissue organization. To reach a better understanding of the mechanics and dynamics of the cortex, we study here HeLa cells in mitosis as a simple model system. In our assay, single rounded cells are dynamically compressed between two parallel plates. Our measurements indicate that the cortical layer is the dominant mechanical element in mitosis as opposed to the cytoplasmic interior. To characterize the time-dependent rheological response, we extract a complex elastic modulus that characterizes the resistance of the cortex against area dilation. In this way, we present a rheological characterization of the cortical actomyosin network in the linear regime. Furthermore, we investigate the influence of actin cross linkers and the impact of active prestress on rheological behavior. Notably, we find that cell mechanics values in mitosis are captured by a simple rheological model characterized by a single timescale on the order of 10 s, which marks the onset of fluidity in the system.

Details

Original languageEnglish
Pages (from-to)589-600
Number of pages12
JournalBiophysical journal
Volume111
Issue number3
Publication statusPublished - 9 Aug 2016
Peer-reviewedYes
Externally publishedYes

External IDs

PubMedCentral PMC4982928
Scopus 84982872935
ORCID /0000-0002-2433-916X/work/142250437

Keywords

Keywords

  • Biomechanical Phenomena, Cell Membrane/metabolism, Elasticity, HeLa Cells, Humans, Mitosis, Rheology, Viscosity