Resistance of L132 lung cell clusters to glyoxal-induced apoptosis

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • C Roehlecke - , Institute of Anatomy (First author)
  • A K Kuhnt - , TUD Dresden University of Technology (Author)
  • H Fehrenbach - , Medical Faculty Carl Gustav Carus (Author)
  • C Werner - , Chair of Biofunctional Polymer Materials (Author)
  • Richard H.W. Funk - , TUD Dresden University of Technology (Author)
  • M Kasper - , TUD Dresden University of Technology (Author)

Abstract

Glyoxal is a highly reactive glycating agent involved in the formation of advanced glycation end products (AGEs) and known to induce apoptosis. AGE-mediated apoptosis may be an important mechanism of alveolar epithelial remodelling in pulmonary fibrosis. In this study, we investigated the cytotoxic effect of glyoxal on the fetal human epithelial lung cell line L132 under serum-free conditions. This type of culture, which forces the cells to grow as spheroids, also excludes effects of preformed AGEs by the reaction of glyoxal with fetal calf serum proteins. Our results showed that in cells treated with 200 microM glyoxal, the intercellular contacts in spheroids were disrupted, i.e. cells became totally dissociated. Immunocytochemical analysis revealed a dose-dependent accumulation of the AGE product epsilonN-(carboxymethyl)lysine (CML) in cells detached from cell clusters. The loss of cell attachment was associated with decreased expression of beta1-integrins and CD44 as revealed by laser scanning cytometry (LSC). Increasing concentrations of glyoxal induced an increase in the number of apoptotic cells which were identified by the immunoreactivity for active caspase-3. Remaining cell clusters showed resistance to both CML formation and apoptosis. The present findings demonstrate that cells treated with glyoxal undergo possibly anoikis, a specific mode of apoptosis caused by loss of cell adhesion.

Details

Original languageEnglish
Pages (from-to)283-92
Number of pages10
JournalHistochemistry and cell biology
Volume114
Issue number4
Publication statusPublished - Oct 2000
Peer-reviewedYes

External IDs

Scopus 0033769093
ORCID /0000-0003-0189-3448/work/159607214

Keywords

Keywords

  • Anoikis/drug effects, Apoptosis/drug effects, Caspase 3, Caspases/metabolism, Cell Line, Dose-Response Relationship, Drug, Drug Resistance, Epithelial Cells/cytology, Glycation End Products, Advanced/metabolism, Glyoxal/pharmacology, Humans, Hyaluronan Receptors/metabolism, Integrin beta1/metabolism, Lung/cytology, Lysine/analogs & derivatives, Spheroids, Cellular/drug effects