Regression and persistence: Remodelling in a tissue engineered axial vascular assembly
Research output: Contribution to journal › Research article › Contributed › peer-review
Contributors
Abstract
In later stages of vasculoangiogenesis a vascular network is going through a metamorphosis for optimal perfusion and economy of energy. In this study we make a quantitative approach to phenomena of remodelling in a bioartificial neovascular network and suggest variance of calibre as a parameter of neovascular maturation. For this study, 18 male Lewis rats were subjected to the AV loop operation in combination with a hard porous biogenic matrix and an isolation chamber. The animals were allocated into three groups for different explantation intervals set to 2, 4 and 8 weeks, respectively. Collective attributes like vascular density, percent fractional area and variance of calibre were evaluated for a predefined region of interest (ROI). Late morphogenesis was evaluated by means of scanning electron microscopy. After the fourth week the absolute number of vessels within the ROI decreased (P < 0.03) whereas, on the contrary, the fractional area of all segments increased (P < 0.02). The variance in calibre was significantly increased in the 8-week group (P < 0.05). Lymphatic growth after week 4, early pericyte migration as well as intussusceptive angiogenesis were identified immunohistologically. Phenomena of remodelling were evaluated quantitatively in a neovascular network and variance could be proposed as a parameter of net vascular maturation.
Details
Original language | English |
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Pages (from-to) | 4166-4175 |
Number of pages | 10 |
Journal | Journal of Cellular and Molecular Medicine |
Volume | 13 |
Issue number | 10 |
Publication status | Published - Oct 2009 |
Peer-reviewed | Yes |
External IDs
PubMed | 19555425 |
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ORCID | /0000-0003-4633-2695/work/145698748 |
Keywords
ASJC Scopus subject areas
Keywords
- Angiogenesis, Arteriovenous loop, Remodelling, Tissue engineering, Vascular regression