RBE of 10 kV X rays determined for the human mammary epithelial cell line MCF-12A

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • Anna Lehnert - , Helmholtz-Zentrum Dresden-Rossendorf (Author)
  • Wolfgang Dörr - , Department of Radiation Oncology (Author)
  • Elisabeth Lessmanna - , Helmholtz-Zentrum Dresden-Rossendorf (Author)
  • Jörg Pawelke - , Helmholtz-Zentrum Dresden-Rossendorf (Author)

Abstract

The dependence of relative biological effectiveness (RBE) on photon energy is a topic of extensive discussions. The increasing amount of in vitro data in the low-energy region indicates this to be a complex dependence that is influenced by the end point and cell line studied. In the present investigation, the RBE of 10 kV X rays (W anode) was determined relative to 200 kV X rays (W anode, 0.5 mm copper filter) for cell survival in the dose range 1-10 Gy and for induction of micronuclei in the range 0.5-3.6 Gy for MCF-12A human mammary epithelial cells. The RBE for cell survival was found to increase with decreasing dose, being 1.21 ± 0.03 at 10% survival. Considerably higher values were obtained for micronucleus induction, where the RBEM obtained from the ratio of the linear coefficients of the dose-effect curves was 2.6 ± 0.4 for the fraction of binucleated cells with micronuclei and 4.1 ± 1.0 for the number of micronuclei per binucleated cell. These values, together with our previous data, support a monotonic increase in RBE with decreasing photon energy down to the mean energy of 7.3 keV used in the present study.

Details

Original languageEnglish
Pages (from-to)330-336
Number of pages7
JournalRadiation research
Volume169
Issue number3
Publication statusPublished - Mar 2008
Peer-reviewedYes

External IDs

PubMed 18302480