Rapid Testing of Gene Function in Axonal Regeneration After Spinal Cord Injury Using Larval Zebrafish

Research output: Contribution to book/conference proceedings/anthology/reportChapter in book/anthology/reportContributedpeer-review

Contributors

Abstract

Larval zebrafish show axonal regrowth over a complex spinal injury site and recovery of function within days after injury. Here we describe a simple protocol to disrupt gene function in this model using acute injections of highly active synthetic gRNAs to rapidly detect loss-of-function phenotypes without the need for breeding.

Details

Original languageEnglish
Title of host publicationAxon Regeneration
EditorsAva J. Udvadia, James B. Antczak
PublisherHumana Press
Pages263-277
Number of pages15
ISBN (print)978-1-0716-3011-2
Publication statusPublished - 2023
Peer-reviewedYes

Publication series

SeriesMethods in Molecular Biology
Volume2636
ISSN1064-3745

External IDs

PubMed 36881306

Keywords

Sustainable Development Goals

ASJC Scopus subject areas

Keywords

  • Axon regeneration, CRISPR/Cas9 mutagenesis, Spinal cord injury, Zebrafish larvae, Axons, Larva/genetics, Phenotype, Animals, Spinal Cord Injuries/genetics, Zebrafish/genetics