Rapid Testing of Gene Function in Axonal Regeneration After Spinal Cord Injury Using Larval Zebrafish
Research output: Contribution to book/Conference proceedings/Anthology/Report › Chapter in book/Anthology/Report › Contributed › peer-review
Contributors
Abstract
Larval zebrafish show axonal regrowth over a complex spinal injury site and recovery of function within days after injury. Here we describe a simple protocol to disrupt gene function in this model using acute injections of highly active synthetic gRNAs to rapidly detect loss-of-function phenotypes without the need for breeding.
Details
| Original language | English |
|---|---|
| Title of host publication | Axon Regeneration |
| Editors | Ava J. Udvadia, James B. Antczak |
| Publisher | Humana Press |
| Pages | 263-277 |
| Number of pages | 15 |
| ISBN (print) | 978-1-0716-3011-2 |
| Publication status | Published - 2023 |
| Peer-reviewed | Yes |
Publication series
| Series | Methods in Molecular Biology |
|---|---|
| Volume | 2636 |
| ISSN | 1064-3745 |
External IDs
| PubMed | 36881306 |
|---|
Keywords
Research priority areas of TU Dresden
DFG Classification of Subject Areas according to Review Boards
Sustainable Development Goals
ASJC Scopus subject areas
Keywords
- Axon regeneration, CRISPR/Cas9 mutagenesis, Spinal cord injury, Zebrafish larvae, Axons, Larva/genetics, Phenotype, Animals, Spinal Cord Injuries/genetics, Zebrafish/genetics