Radiation-induced variations in urothelial expression of intercellular adhesion molecule 1 (IGAM-1): Association with changes in urinary bladder function

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • J. Jaal - , Tartu University Clinics, Department of Radiation Oncology (Author)
  • K. Brüchner - , Experimental Center of the Faculty of Medicine (Author)
  • C. Hoinkis - , Tartu University Clinics (Author)
  • W. Dörr - , Department of Radiation Oncology (Author)

Abstract

Purpose: To assess the effect of single-dose irradiation on intercellular adhesion molecule 1 (ICAM-1) expression in the urothelium of mouse urinary bladder and to correlate ICAM-1 variations with fluctuations in storage capacity during the early and late radiation response. Materials and methods: Groups of female C3H mice were subjected to irradiation with either 20 or 0 Gy. The intensity of immunohistochemical ICAM-1 staining in the urothelium was assessed in a semiquantitative way applying an arbitrary score (0-5). Changes in bladder storage function were assessed by transurethral cystometry. Results: For the early radiation response phase, a reduction in bladder capacity by >50%, i.e. a positive functional radiation response, was seen in 40% of the irradiated animals between days 0 and 15, and in 64% of animals during days 16-30. During the late response phase, 71% of the animals sacrificed after day 180 developed a positive functional response. Urothelial cells were found to express ICAM-1 constitutively. Irradiation resulted in an early rise in staining signal by day 2, with a maximum on day 4 and a return to control values on day 13. A permanent increase in ICAM-1 staining signal was observed in the late phase, from day 90 to 360 after irradiation. The expression of ICAM-1 in animals with a positive late response was 4.2 ± 1.2 (mean ± standard deviation), compared with 2.6 ± 1.0 in non-responders (p = 0.0009). Conclusion: Irradiation induces significant acute and chronic changes in urothelial ICAM-1 expression indicating that the urothelium contributes to the pathogenesis of both acute and late radiation effects in the urinary bladder.

Details

Original languageEnglish
Pages (from-to)65-72
Number of pages8
JournalInternational journal of radiation biology
Volume80
Issue number1
Publication statusPublished - Jan 2004
Peer-reviewedYes

External IDs

PubMed 14761851