Quantifying the effect of covalently immobilized enzymes on biofilm formation by atomic force microscopy-based single-cell force spectroscopy

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • Jens Friedrichs - , Leibniz Institute of Polymer Research Dresden (Author)
  • Andrea Zieris - , Leibniz Institute of Polymer Research Dresden (Author)
  • Silvana Prokoph - , Leibniz Institute of Polymer Research Dresden (Author)
  • Carsten Werner - , Chair of Biofunctional Polymer Materials, Leibniz Institute of Polymer Research Dresden (Author)

Abstract

A novel atomic force microscopy-based single-cell force spectroscopy assay to quantify the adhesion of bacterial cells to surfaces was developed. The assay was applied to quantify the effect of two biofilm-degrading enzymes, the protease Subtilisin A and glycoside hydrolase cellulase, on the attachment of the biofilm-forming bacterial strain Cobetia marina. Insights on the mechanism of the initial adhesion and on the nature of the adhesion-mediating molecules were gained. The assay can be easily adapted to various other substrates, different bacterial strains and other fouling species (e.g., algae and diatoms).

Details

Original languageEnglish
Pages (from-to)1453-1458
Number of pages6
JournalMacromolecular rapid communications
Volume33
Issue number17
Publication statusPublished - 14 Sept 2012
Peer-reviewedYes

External IDs

PubMed 22829309
ORCID /0000-0003-0189-3448/work/162347628

Keywords

Keywords

  • atomic force microscopy, biofilm-degrading enzyme, biofouling, single-cell force spectroscopy