Quantification of unperturbed phosphoprotein levels in immune cell subsets with phosphoflow to assess immune signaling in autoimmune disease

Research output: Contribution to journalCase reportContributedpeer-review


  • Calvin Krollmann - , University of Bonn Medical Center (Author)
  • Kevin Cieslak - , University of Bonn Medical Center (Author)
  • Ruth-Miriam Koerber - , University of Bonn Medical Center (Author)
  • Hella Luksch - , Department of Paediatrics (Author)
  • Angela Rösen-Wolff - , Department of Paediatrics (Author)
  • Peter Brossart - , University of Bonn Medical Center (Author)
  • Lino L Teichmann - , University of Bonn Medical Center (Author)


Activation of innate immune sensors by endogenous DNA and RNA can lead to autoimmune and autoinflammatory diseases. Quantification of the unperturbed phosphoprotein content in immune cells provides insight into the spontaneous activity of immune signaling pathways triggered by nucleic acid recognition. Here, we present a phosphoflow protocol for measuring phosphoproteins in mouse models of autoimmunity that incorporates strategies to preserve native phosphoprotein levels during sample collection and to reliably detect low signaling activity common in chronic disease states. For complete details on the use and execution of this protocol, please refer to Jütte et al. (2021).


Original languageEnglish
Article number101309
JournalSTAR Protocols
Issue number2
Publication statusPublished - 17 Jun 2022

External IDs

PubMedCentral PMC9038771
Scopus 85128215921
Mendeley bacb2660-ca23-3b4f-9719-1769e33daf6f



  • Animals, Autoimmune Diseases/genetics, Autoimmunity, Mice, Nucleic Acids, Phosphoproteins, Signal Transduction/physiology, Immunology, Flow Cytometry/Mass Cytometry