Purification of foamy viral particles

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

Abstract

Foamy viruses are non-pathogenic retroviruses and represent a tool for vector development. For gene therapy applications and for analyses of viral protein composition infectious particles need to be purified, which has been difficult for foamy viruses in the past. Here, we describe a novel, simple, and fast purification method for prototype foamy viruses with high purity using size exclusion and affinity chromatography. More than 99,9% of the contaminating proteins were removed. The purified viruses were used to determine the amount of the incorporated Pol protein relative to Gag. The determined Gag to Pol PR-RT ratio of 30:1 confirmed previous studies suggesting FV virions encapsidate fewer number of Pol molecules than orthoretroviruses.

Details

Original languageEnglish
Pages (from-to)28-33
Number of pages6
JournalVirology
Volume506
Publication statusPublished - Jun 2017
Peer-reviewedYes

External IDs

ORCID /0000-0002-0320-4223/work/150885001
Scopus 85015028914

Keywords

Keywords

  • Chromatography, Affinity/methods, Chromatography, Gel/methods, Gene Expression Regulation, Viral, Gene Products, pol/genetics, Humans, Retroviridae Infections/virology, Spumavirus/genetics, Virion/genetics, Virus Assembly, Virus Cultivation