Publication guidelines and recommendations for pupillary measurement in psychophysiological studies

Research output: Contribution to journalEditorial (Lead article)Invitedpeer-review



A variety of psychological and physical phenomena elicit variations in the diameter of pupil of the eye. Changes in pupil size are mediated by the relative activation of the sphincter pupillae muscle (decrease pupil diameter) and the dilator pupillae muscle (increase pupil diameter), innervated by the parasympathetic and sympathetic branches, respectively, of the autonomic nervous system. The current guidelines are intended to inform and guide psychophysiological research involving pupil measurement by (1) summarizing important aspects concerning the physiology of the pupil, (2) providing methodological and data-analytic guidelines and recommendations, and (3) briefly reviewing psychological phenomena that modulate pupillary reactivity. Because of the increased ease and tractability of pupil measurement, the goal of these guidelines is to promote accurate recording, analysis, and reporting of pupillary data in psychophysiological research.


Original languageEnglish
Article numbere14035
Pages (from-to)1-36
Number of pages36
Issue number4
Publication statusPublished - 22 Mar 2022

External IDs

Scopus 85126837117
Mendeley 74985e09-fd4d-3033-950b-80fa1b3769e1
WOS 000771764700009
PubMed 35318693
unpaywall 10.1111/psyp.14035


DFG Classification of Subject Areas according to Review Boards


  • dilation, parasympathetic, pupil constriction, pupillography, pupillometry, sympathetic, Reflex, Pupillary/physiology, Humans, Autonomic Nervous System, Pupil/physiology, Psychophysiology

Library keywords