Programmable synthetic cell networks regulated by tuneable reaction rates
Research output: Contribution to journal › Research article › Contributed › peer-review
Contributors
Abstract
Coupled compartmentalised information processing and communication via molecular diffusion underpin network based population dynamics as observed in biological systems. Understanding how both compartmentalisation and communication can regulate information processes is key to rational design and control of compartmentalised reaction networks. Here, we integrate PEN DNA reactions into semi-permeable proteinosomes and characterise the effect of compartmentalisation on autocatalytic PEN DNA reactions. We observe unique behaviours in the compartmentalised systems which are not accessible under bulk conditions; for example, rates of reaction increase by an order of magnitude and reaction kinetics are more readily tuneable by enzyme concentrations in proteinosomes compared to buffer solution. We exploit these properties to regulate the reaction kinetics in two node compartmentalised reaction networks comprised of linear and autocatalytic reactions which we establish by bottom-up synthetic biology approaches.
Details
Original language | English |
---|---|
Article number | 3885 |
Journal | Nature communications |
Volume | 13 |
Issue number | 1 |
Publication status | Published - 6 Jul 2022 |
Peer-reviewed | Yes |
External IDs
PubMed | 35794089 |
---|